The Basic Principles Of flow cytometer

The Basic Principles Of flow cytometer

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Facts-enriched characterization of mobile populations. Morphological data from images can increase for the richness of flow cytometry details. For instance, the determine reveals an otherwise conventional apoptosis assay employing Annexin V and PI, introducing cell imaging to characterize cells in Each individual population to expose morphologically distinct characteristics. These insights couldn't are already obtained from multiplex staining on your own.

As samples are acquired over the Attune CytPix Flow Cytometer, the large-velocity brightfield camera captures and shops photos of detected occasions at a rate approximately six,000 photographs for every second, dependant upon the flow amount and image measurement. For higher adaptability, Attune Cytometric Software program helps you to alter the picture capture frequency as wanted and choose a specific gate for impression seize, if essential.

Mix acoustic focusing technologies with significant-parameter Assessment for flexible spectral unmixing and traditional workflows.

The award-profitable Invitrogen Attune CytPix Flow Cytometer brings together acoustic focusing by using a higher-pace brightfield digicam for simultaneous superior throughput flow cytometry and significant resolution brightfield imaging.

*Pricing is indicative only and is subject to vary. You should Get hold of your Agilent agent with any inquiries. The knowledge set forth herein will not be a proposal or perhaps a motivation, equally of that happen to be issue to credit history approval, terms and conditions, and availability with Agilent. Agilent Fiscal Answers is offered in selected nations around the world.

Here, a highly skilled person has gated singlets confidently. Just after assessing the manual singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate consists of more than four% aggregates.

Pair your Attune flow cytometer with the autosampler for elevated workflow effectiveness, and include robotic integration for optimum throughput.

Steady image top quality even at high throughput Acoustic focusing as well as a high-pace camera Mix to image these T cells continually at lower or superior flow costs. Very easily regulate focus and digicam configurations to meet experimental needs.

The Attune NxT cytometer will be the trusted workhorse instrument your lab can trust in for dependable and trustworthy performance. The Attune CytPix product encompasses a higher-speed brightfield camera and AI-pushed graphic Evaluation software package, with recent updates including equipment Discovering tools and an intuitive person interface, enabling Increased facts precision, accuracy, and quicker insights into sample biology.

Customers can look at party images in true-time to substantiate sample top quality, improve protocols, and file the morphology of each cell inhabitants discovered for Assessment. Imaging capture also Positive aspects from acoustic concentrating, which helps you to place the cells so that a sharp, centered image is received.

Sound waves allow specific cell alignment, leading to Excellent details top quality and ~10X faster sample flow premiums.

Application Take note: Automatic graphic Assessment minimizes consumer-to-consumer variability in flow cytometry gating strategies

Developed-in dimensionality reduction resources increased resolution into populations. Fluorescently gated populations back-gated onto UMAP plot to here verify predicted population resolution in lysed complete human blood. Person can check out functions from Each individual cluster for Visible confirmation of mobile phenotypes.

No wash/no lyse leukocyte analysis with imaging. Cells were acquired from 24-hour-outdated blood by dilution in 1 mM EDTA ( Qualified UX/UI permits users to prepare AI styles for expanded applications. (A) The “Educate Design” feature provides annotation tools and true-time product instruction accuracy.

Gating on these extended parameters allows you to promptly and properly discover populations of desire and confirm gating approach with little or no manual overview. The Attune program provides access to state-of-the-artwork equipment Understanding Assessment applications within just an expertly created person interface, rendering it extremely simple to operate.

The Attune Xenith cytometer can be a significant-parameter spectral analyzer, delivering both equally spectral unmixing and traditional flow capabilities for extensive and flexible cell analysis.

Agilent Price Assure assures you at the least ten years of instrument use with the day you buy. Or else, We are going to credit rating you with the residual value of the procedure towards an upgraded design.

Here, we display that by gating procedures employing graphic-based mostly quantitative parameters can distinguish interacting cells from coincident activities to a lot more precisely review interacting cells.